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straight40lkXV tory burch sale 2 Methods and results
2 Methods and results

1 equipment and materials

TU-1901 double beam UV-visible spectrophotometer (Beijing General), vanillin (3 - methoxy -4-- hydroxybenzaldehyde), anisaldehyde (p-methoxy benzaldehyde), dimethylamino benzaldehyde, ethanol, methanol, sulfuric acid, are AR. level. SAaB A Ⅲ standard (Shanghai Second Military Medical University, Department of Pharmacy offers high performance liquid normalization method measured 97.2% purity) Anemarrhenae extract, a total of nine batches (060512,060515,060521,060622,060728 , 060808,060816,060908,060928), the extraction and isolation of their own laboratory.

2.7 precision experiments at The results show that good reproducibility of the method, RSD less than 2.0%.

Determination of total saponin anemarrhena

Abstract OBJECTIVE To determine Anemarrhenae extract content of steroidal saponins in the colorimetric method. Methods to do with anisaldehyde sulfuric acid color reaction,mercurial vapor iv fg, detection wavelength 430nm, the optimum experimental conditions. Results The standard curve was 25 ~ 150μg good linear relationship,mercurial vapor superfly 7cF5The new Nike Mercurial - 98 Roc, correlation coefficient 0.999 8, average recovery 98.2% (RSD = 3.18%). Conclusion The good stability and reproducibility of the method can be used to extract of steroidal saponins Anemarrhena substances purity test.

Anemarrhena lily plants Zhimu Anemarrhena asphodeloides Bge. Rhizome dry, cold, bitter sweet, with heat purging fire, the effect of moistening fluid for the exogenous fever, high fever, polydipsia, dry Hyperactivity Well, Gu Zheng, hot flashes, heat diabetes, constipation,mercurial vapors, dry bowel [1]. Anemarrhena steroidal saponin is one of the main active ingredient. Pharmacological studies have shown that steroidal saponin Anemarrhena lower blood pressure, Currently, the determination of total saponins anemarrhena methods include colorimetry. In this paper, colorimetry, the different color system were studied to determine timosaponin extract substances in the saponin content of the extract Zhimu provide reference for quality control.

2.4 precise color temperature control solution were drawn 8, 100 μl, to a test tube with a plug, the solvent evaporated to dryness, remove,mercurial rose 4fM8Coupe du monde et chaussures Ni, respectively, 0.5% anisaldehyde sulfuric acid 0.5 ml, 2 copies from each of at 25 ℃, 60 ℃, 80 ℃ and 100 ℃ and reaction 15 min,tory burch sale, removed and immediately placed in ice bath home 5 min,mbt scarpe italia, precision anhydrous methanol, 10 ml, shake, respectively, the temperature of the reagent blank tube determine the maximum absorption wavelength and absorbance. The results showed that the reaction temperature had no effect on the maximum absorption wavelength are 430 nm and 504 nm in wavelength of maximum absorption, but as the temperature increases, 430 nm absorbance decrease at, 504 nm absorption at higher,abercrombie deutschland, while in 100 ℃, the highest absorption, more sensitive, so choose the reaction temperature is 100 ℃, detection wavelength 430 nm.

2.5 color time of 6 copies of the reference solution precision drawing 100 μl, the reaction temperature under 100 ℃, reaction time 5,10,mercurial 4iZsakrabyewex - Fifa 11 - top ten goals,15 min on the impact absorption, and stability of absorption ######. The results in Table 2. The results showed that, 100 ℃ 5 min reaction higher absorbance, 30 min in the stability is good, so choose under 100 ℃, reaction time 5 min is appropriate. Table 2, reaction time and the impact on the stability of the color (slightly)

2.1 reference solution accurately weighed amount of SAaB A Ⅲ reference substance, add methanol solution and produced containing about 0.5 mg per ml of solution, as the reference solution.

Key words anemarrhenae; colorimetry; steroidal saponins; anisaldehyde

2.6 linear range of precision drawing reference solution were investigated 50,100,150,200,250,300 μl, to a test tube with a plug, the solvent evaporated to dryness, remove, respectively, 0.5% anisaldehyde sulfuric acid solution 0.5 ml, the reaction at 100 ℃ for 5 min, removed and immediately placed in ice bath home 5 min, precision anhydrous methanol, 10 ml, shake to reagent blank tube, was measured at 430 nm absorbance wavelength in order to control product addition is horizontal,Tory Burch Reva Sale, vertical coordinates for the absorbance, calculate the regression equation. The results showed that timosaponin A Ⅲ in the range of 25 ~ 150 μg, linear regression equation Y = 3.745 8X-0.037 7 (Y is the absorbance, X is the number of reference substance mg), r = 0.999 8.

2.3 reagent to study the best choice of color reagent, the reference literature [7] investigated vanillin, anisaldehyde, dimethylamino benzaldehyde reagent such as the effectiveness and stability of the Colour nature and amount of preparation methods are:

A: 1% vanillin in ethanol solution 1 ml + sulfuric acid - ethanol (7 → 10) 1 ml;
;
B: 0.5% anisaldehyde sulfuric acid 0.5 ml (0.1ml anisaldehyde sulfuric acid +1 ml +18.9 ml ethanol);

C: 0.35 mol / L of dimethyl amino benzaldehyde solution 1ml + 0.08 mol / L hydrochloric acid ethanol, 1 ml;

method: precision drawing standard solutions, the test solution of each 100 μl, set 10 ml stoppered test tube, the solvent evaporated to dryness, remove, respectively, by the above method by adding a different reagent, shake evenly, home heating at 100 ℃ 5 min, removed and immediately placed in ice bath home 5 min,ralph lauren sale, each tube Precision anhydrous methanol 10 ml, shake, respectively, the same with the color reagent blank tube, according to spectrophotometry (. Tested once every 5 min, 30 min observation of the stability of the absorption. The results in Table 1. Table 1 Several reagent on the color effect and stability of the (slightly)

results show that vanillin as coloring agent was less stable, dimethylamino benzene Formaldehyde is not color, so choose to anisaldehyde as the color reagent, and further investigation of various factors on the impact of color.

2.8 Determination of Precision Weigh Anemarrhena extract about 100 mg, set 50 ml flask, add methanol dissolved and diluted to volume, shake, precision drawing 100 μl, determined by standard curve, Calculation of saponin. The results in Table 3. Table 3 Anemarrhena extract assay results (omitted)

2.2 for the test sample solution, accurately weighed Anemarrhena extract powder 100 mg, set 50 ml flask, add methanol dissolved and diluted to the scale, made of 2 mg per ml of solution containing about as the test solution.
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